英国医学研究理事会DNA复制机制分析博士后岗位
Postdoctoral Research Fellow : London, W12 0NN, United Kingdom
Employer
Medical Research Council
Website
Location
London, W12 0NN, United Kingdom
Posted
August 27, 2009
Expires
September 28, 2009
Jobs by tag(s)
dna replication, biochemistry, biophysical, biology, postdoctoral research fellow, career development fellow, clinical sciences centre
Description
Postdoctoral Research Fellow
Biochemistry, Molecular Biology & Structural Biology:
Analysis of the DNA Replication machinery
MRC Band 4 Salary £29,663 – £35,399 per annum (including location allowances)
London W12
Fixed-term 2 Year Post with possibility for extension
The Clinical Sciences Centre, which is directly funded by the Medical Research Council, is a division of Imperial College. The CSC and Imperial College provide excellent core facilities and support structures. The DNA Replication Laboratory is part of the Epigenetics, Development and Cancer Section and offers interactions with groups studying cell cycle, cancer, nuclear organisation and chromatin.
We are looking to recruit a Postdoctoral Research position within the DNA Replication Group at the MRC-CSC. We are looking for a very motivated Biochemist that is interested in joining our friendly and international team. You will join a rapidly developing research project focussed on the eukarotic MCM2-7 helicase. We have previously characterised the assembly process of the ORC-Cdc6 complex on DNA (Speck et al. 2005 Nature Structural & Molecular Biology; Speck and Stillman 2007 JBC; Speck et al 2008 PNAS).
Recently, we purified the MCM2-7 helicase and showed that MCM is active in pre-Replication Complex formation. Now we aim to characterise its helicase activity alone and in combination with pre-Initiation Complex (pre-IC) proteins. Furthermore, we want to understand the influence of S-phase specific kinases on helicase activity. Finally, the replication fork architecture and activities associated with the replication fork will be investigated. Newly identified mechanisms will be verified in vivo using a mutagenesis approach. In addition, we will investigate the structure of the MCM2-7 complex in the context of the pre-IC using electron microscopy. Methods include biochemical and biophysical approaches and structural work in an ongoing collaboration with a Cryo EM lab.
You should be highly motivated, committed and have clear career goals. A strong background in biochemistry and protein purification is required. The candidate would benefit from experience in analysis of protein-DNA interactions. You should have a PhD in Biochemistry, Biology or equivalent experience and at least one first-author publication.
Contact
For further information including a detailed job description and application form please see:
http://www.csc.mrc.ac.uk/Jobs/Vacancies/
For informal enquiries regarding the post please contact Dr Christian Speck (christian.speck@csc.mrc.ac.uk)
DNA Replication Group page: http://www.csc.mrc.ac.uk/Research/Groups/EDC/DNAReplication/
http://dnareplication.csc.mrc.ac.uk/
If you would like to receive this advert in large print, Braille, audio, or electronic format/ hard copy, please contact the Recruitment team at the MRC Shared Service Centre on the telephone number below or recruitment@ssc.mrc.ac.uk.
Applications for this role should be made online at http://jobs.mrc.ac.uk. If you do not have internet access or experience technical difficulties please call +44 (0)1793 301154 quoting the reference number CSC09/444. Please ensure you attach a copy of your CV and covering letter.
Closing date for applications is 28 September 2009 .