辛辛那提儿童医院研究基金发育和再生神经生物学研究博士后岗位
来源：丁香人才网
日期：2008-04-01
Institution
Cincinnati Children's Hospital Research Foundation
Position
Postdoctoral Fellow in Developmental and Regenerative Neurobiology
Location
Cincinnati, OH, USA
Date Posted
March 31th 2008
Date Expires
April 30th 2008
Tags
postdoctoral fellow, developmental and regenerative neurobiology,cincinnati, oh, and usa
Description
We are using C. elegans as a model system to study cellular andmolecular mechanisms underlying neuronal development and
regeneration. The C. elegans nervous system is composed of 302 neuronswith a complete map of all axon trajectories and
synaptic connections. The transparency and small size of C. elegansallows us to target any axon with femtosecond laser
ablation, as well as visualize axonal development and axonalregeneration in live animals using time-lapse fluorescence
microscopy. Femtosecond laser ablation is a new optical scalpel withexquisite precision and reproducibility. The nanometer
precision of femtosecond laser ablation, as well as the million-foldshorter exposure interval, allows us to snip
individual nerve fibers without collateral damages to the cell body orneighboring fibers. C. elegans has very powerful
genetic tools to illustrate basic biological mechanisms as exemplifiedby C. elegans biologists winning 2002 Nobel Prize in
programmed cell death and again past year Nobel Prize in RNAi. We thushave a unique opportunity to apply powerful genetic
and optical approaches in C. elegans to dissect brain wiring andrewiring mechanisms.
References:
Gabel, CV, Antonie, F, Chuang, C-F, Samuel, AD, and Chang, C. Distinctcellular and molecular mechanisms mediate initial
axon development and adult-stage axon regeneration in C. elegans.Development (2008) 135: 1129-1136.
Chang C, Adler C, Krause M, Clark S, Hao J, Gertler F, Tessier-LavigneM, Bargmann CI.
MIG-10/Lamellipodin and the lipid modulator AGE-1/PI3K promote axonguidance and outgrowth in response to Slit and Netrin.
Current Biology (2006) 16: 1-9.
Samuel A, Chung SH, Clark DA, Gabel CV, Chang C, Murthy V and Mazur E.Femtosecond laser dissection in C. elegans neural
circuits. Proceedings of The International Society for OpticalEngineering (2006) 6108: 1-6.
Chang C, Yu TW, Bargmann CI, Tessier-Lavigne M.
Inhibition of Netrin-mediated axon attraction by a receptor proteintyrosine phosphatase.
Science (2004) 305: 103-106.
Qualifications:
Prior experience in C. elegans is helpful but not essential. Applicantsshould have a Ph.D. in genetics, cell biology,
molecular biology, neurobiology, or biochemistry and are familiar withmolecular biology techniques.
Contact
Interested candidates please send a brief cover letter describingresearch experience, CV, and names and contact
information of two references to Dr. Chieh Chang at chieh.chang@mcgill.ca.
Cincinnati Children's Hospital Research Foundation
Position
Postdoctoral Fellow in Developmental and Regenerative Neurobiology
Location
Cincinnati, OH, USA
Date Posted
March 31th 2008
Date Expires
April 30th 2008
Tags
postdoctoral fellow, developmental and regenerative neurobiology,cincinnati, oh, and usa
Description
We are using C. elegans as a model system to study cellular andmolecular mechanisms underlying neuronal development and
regeneration. The C. elegans nervous system is composed of 302 neuronswith a complete map of all axon trajectories and
synaptic connections. The transparency and small size of C. elegansallows us to target any axon with femtosecond laser
ablation, as well as visualize axonal development and axonalregeneration in live animals using time-lapse fluorescence
microscopy. Femtosecond laser ablation is a new optical scalpel withexquisite precision and reproducibility. The nanometer
precision of femtosecond laser ablation, as well as the million-foldshorter exposure interval, allows us to snip
individual nerve fibers without collateral damages to the cell body orneighboring fibers. C. elegans has very powerful
genetic tools to illustrate basic biological mechanisms as exemplifiedby C. elegans biologists winning 2002 Nobel Prize in
programmed cell death and again past year Nobel Prize in RNAi. We thushave a unique opportunity to apply powerful genetic
and optical approaches in C. elegans to dissect brain wiring andrewiring mechanisms.
References:
Gabel, CV, Antonie, F, Chuang, C-F, Samuel, AD, and Chang, C. Distinctcellular and molecular mechanisms mediate initial
axon development and adult-stage axon regeneration in C. elegans.Development (2008) 135: 1129-1136.
Chang C, Adler C, Krause M, Clark S, Hao J, Gertler F, Tessier-LavigneM, Bargmann CI.
MIG-10/Lamellipodin and the lipid modulator AGE-1/PI3K promote axonguidance and outgrowth in response to Slit and Netrin.
Current Biology (2006) 16: 1-9.
Samuel A, Chung SH, Clark DA, Gabel CV, Chang C, Murthy V and Mazur E.Femtosecond laser dissection in C. elegans neural
circuits. Proceedings of The International Society for OpticalEngineering (2006) 6108: 1-6.
Chang C, Yu TW, Bargmann CI, Tessier-Lavigne M.
Inhibition of Netrin-mediated axon attraction by a receptor proteintyrosine phosphatase.
Science (2004) 305: 103-106.
Qualifications:
Prior experience in C. elegans is helpful but not essential. Applicantsshould have a Ph.D. in genetics, cell biology,
molecular biology, neurobiology, or biochemistry and are familiar withmolecular biology techniques.
Contact
Interested candidates please send a brief cover letter describingresearch experience, CV, and names and contact
information of two references to Dr. Chieh Chang at chieh.chang@mcgill.ca.
[作者：jurgen 编辑：]