博士后(中美联合梅毒研究项目)

1、Job Description
Position:Postdoctoral Researche
Specialization:Pathogenic Microbiology, Molecular Biology, StructuralBiology, etc.
Minimum Educational Qualification: Ph.D.
Work Experienc:No specific requirement, candidates with experiencestudying in the United States are preferred

2、International Exchange
During the station period, you can conduct research projects at theSpirochete Research Laboratories of the University of Connecticut,Connecticut Children’s - Division of Infectious Diseases, and UConnHealth - Departments of Pediatrics and Medicine, under the guidance ofProf. Justin Radolf.
在站期间可前往美国康涅狄格大学Spirochete Research Laboratories Connecticut Children’s -Division of Infectious Diseases & UConn Health - Departments ofPediatrics and Medicine实验室在Prof. Justin Radolf指导下进行课题研究。


3、Job Responsibilities
Participate in the collaborative project between the National Institutesof Health (NIH) and China, titled "Global sequence and surfaceantigenic diversity of Treponema pallidum outer membrane proteins"(UCHC7-122226512). Specifically, conduct research on the development ofnanovaccines by combining single-cell omics sequencing technologies andbio-tracing techniques to screen for syphilis disease progressionbiomarkers and protective antibodies.
Familiarity with clinical aspects of syphilis and its immunopathogenicmechanisms,as well as foundational experiments and animal experimentsrelated to syphilis.
Prokaryotic expression and purification of key ECL (extracellular loop)of Treponema pallidum candidate proteins.
Detection of the reaction between key ECL and antibodies from differentpatient sera.
Use bioinformatics tools to analyze the genomic polymorphism ofTreponema pallidum, focusing on the variation of outer membraneproteins.
Preparation of monoclonal antibodies from BCR (B cell receptor)sequences of skin lesions.
Screen and validate antibodies obtained from the existing syphilisprotein library. Use the obtained antibodies to screen, identify, andanalyze unknown syphilis proteins.
Other research related to syphilis.

4、Job Requirements
Ph.D. graduate in relevant majors such as pathogenic microbiology,molecular biology, structural biology, etc.
Experience in recombinant protein expression using expression systemslike Escherichia coli and mammalian cells, as well as related work inexpression system selection, purification, antigen/antibody production,and identification. Experience in the study of bacterial outer membraneproteins is preferred.
Basic understanding of bioinformatics.
Strong research literacy, ability to plan and execute experiments, solveexperimental problems, and analyze results.
Strong teamwork, communication, coordination, and expression skills.